Capillary electrophoresis for the analysis of large biomolecules and molecular assemblies (proteins, DNAs, phages, viruses)

• Investigation of binding constants and stochiometry of protein/ligand systems by CE.

• Study of the migration modes of polyelectrolytes like DNAs in solutions with sieving properties.

• Investigation of large assemblies like phages, viruses and their denaturation product by capillary isoelectric focusing.

Effect of organic buffer additives on the electrophoretic behavior of analytes 

• Investigation of the effect of organic buffer additives (organic solvents, polymers or other additives) on the migration behavior of separands in capillary electrophoresis changing (i) the mobility of the fully charged ion, and (ii) the acidity or basicity of the analytes and the separation buffers.

• Peak efficiency in dependence of the solvent, especially effects on diffusion and self-heating.

• Investigation of organic buffer additives on pI values of zwitterionic assemblies like proteins by capillary isoelectric focusing.

Theoretical description of electrically driven methods

• Description of zone migration and dispersion processes (with plate height concept) based on their physico-chemical principles.

•  Electric double layer and zeta potential.

Application of electromigration methods in the  capillary format (CE, MEKC, MEEKC, CEC) to pharmaceuticals, food and feed analysis.

• Determination of bioactive compounds, food and feed constituents by electrically driven separation methods by different separation principles. 

• Use of isotachophoresis as preconcentration step for the following on-line capillary zone electrophoretic determination.

Natural binding media in museum objects

• Characterization and identification of natural organic binding media applied in objects of relevance for history of art and culture by electrically driven separation methods: animal glues (proteins), resins, plant gums (polysaccharides), drying oils.

• Application of capillary GC with various detection schemes (FID, ECD, FPD, MS) to the analysis of natural binding media of museum objects.